|10:00 am||- Gautam Subramanian||(Dr. Hussam)|
|Development of Arduino based pH titration system for identification of common drugs|
|10:20 am||- Sanae Moummi||(Dr. Paige)|
|A Simplified Method of Sonogashira Coupling|
|10:40 am||- Silvia Auffret||(Dr. Paige)|
|Synthesis of unsymmetrical bis-alkynes|
|11:00 am||- Mosufa Zainab||(Dr. Couch)|
|Development of Novel Antibiotics Targeting the MEP Pathway|
|11:20 am||- Benjamin McDowell||(Dr. Schreifels)|
|Electrospray Ionization in Mass Spectrometry for the Analysis of Volatiles|
|11:40 am||- Brittany Rapp||(Dr. Hussam)|
|Dynamics of Gold Nanoparticle Formation with Cyclohexanone as Reducing Agent at Room Temperature|
|12:30 pm||- Lunch||Planetary Hall - Room 312|
|Please be prompt and please sign in for lunch attendance|
Besides Thermodynamics, What Other Factors Are Controlling Mineralization and Weathering at Near-Surface Conditions?
Henry Teng (George Washington University)
Please email Dr. You at firstname.lastname@example.org if you’d like to meet the speaker.
Natural process of mineral formation and dissolution at near-surface conditions are subject to various effects ranging from that of aqueous speciation to biological participation. As such complications arise for laboratory investigations of mineralization and weathering when thermodynamicd is considered the only driving force. Two case studies, one is solution chemistry effect on crystallization and the other microbially mediated dissolution, will be discussed in this presentation to highlight the complexity.
For mineralization, the classical approach states that the net growth rate of mono-molecular layers (ie, step velocity) is determined by the difference between fluxes of species attaching to and detaching from kinks along step edges. Such treatment leads to the development of the widely accepted understanding that step velocity depends solely on solution supersaturation. Yet, literature data from numerous cases argued strongly against this supposition. In this study, we conducted a series of in situ AFM experiments to interrogate the effect of solution chemistry parameters on step kinetics using calcite as a model system. We found step kinetics were strong affected by solution pH, ionic strength SI, and the [Ca2+]/[CO32-] ratio, and the impact differs in different cleavage directions. These observations suggest that, although supersaturation is the driving force for aqueous phase crystallization, solution chemistry plays critical roles in controlling the actual growth rate and needs to be taken into consideration in kinetic studies of crystallization.
For bio-weathering, complications are often associated with cell-mineral interfacial reactions because of physiology changes in microbes once becoming surface-bound and also because of the involvement of biomechanical forces in the case of fungal dissolution. In this study we examined lizardite [Mg3Si2O5(OH)4] dissolution by a native fungal strain in bulk media and at interface through determining the total metal release in culture, the pH local to surface-bound cells, and the material composition and structure beneath cell-colonized surfaces. We found that (1) cellular dissolution proceeds by a mechanism fundamentally different from that at the mineral-water interface, (2) only attached cells release siderophores, and (3) biomechanical forces of hyphal growth are indispensable for fungal weathering and strong enough to breach the mineral lattice. These results strongly suggest that fungal cell-promoted interfacial dissolution may have been significantly underestimated in the current understanding of microbial geochemistry.
Characterizing the interactions of the B-box E3 ligase with E2 conjugating enzyme: Learning about protein ubiquitination.
Michael Massiah (George Washington University)
Dr. Massiah will present recent work on the B-box domain from the human MID1 protein. The B-box domain may represent a new member of the ubiquitin E3 ligase, with similar fold as the RING-type E3 ligase. However, it has weaker activity. Despite this, it is require for the targeting and polyubiquitination of protein phosphatase 2A (a molecular master switch), alpha4, and the fused kinase. To understand how the B-box domain functions, NMR and fluorescence spectroscopy were used to characterize its interaction with the E2 enzyme, UbcH5, and mutagenesis to identify structural features that are important for ligase activity, and to engineer a more active E3 ligase. He will also discuss interesting observation how RNA affect MID1 E3 ligase activity.